Faculty
 David L. Kaplan
Professor & Chair - Department of Biomedical Engineering
Director, Bioengineering and Biotechnology Center
Professor, Department of Chemical & Biological Engineering
4 Colby Street, Medford, Massachusetts 02155
Telephone: (617) 627-3251 ~ Fax: (617) 627-3231
david.kaplan@tufts.edu
Education:
1975 B.S. Biology, State University of New York at Albany
1978 Doctor of Philosophy, Biology/Biochemistry, State University of New York at Syracuse and Syracuse University
Honors:
- New York State Regents Scholarship (1971-1975)
- Decoration for Meritorious Civilian Service (1990)
- Senior Research Scientist (ST Position) (1995)
- Elected President - Bioenvironmentally Degradable Polymer Society (1996)
- Invited Panel Chair - USDA Grant Review Program,
- Invited Book Series Editor, NIH - Bioengineering Materials, Birkhauser (1996 - Present)
- Outstanding Faculty Award, Tufts University (1998)
- Appointed Associate Editor, Biomacromolecules (1999)
- Editorial Boards - Applied Environmental Microbiology, Bimolecular Materials, Advanced Materials, e-Biomed, Soft Materials (1999)
- Elected Fellow, American Institute of Medical and Biological Engineering
- Director, NIH Resource Center, Tissue Engineering
Research Interests, Areas, or Ongoing Projects:
Research is focused at the interface between biology and materials science and engineering. Our research is aimed at understanding biological synthesis and processing of polymers and polymer interfaces. Issues of structure-function related to self-assembly and macromolecular assembly are key to this understudy. Activities are focused on biomaterials and functional tissue engineering. We approach this problem from three levels:
- Genetic Approach: Exploration of the molecular genetics of biosynthesis pathway for biopolymers.
- Whole Cell Approach: Manipulation of the Cell Environment to regulate the nature of the biopolymer synthesized.
- Enzymatic Approach: Polymer synthesis using enzymes in vitro in novel environments.
Examples of Topics:
- Fibrous Proteins - New Paradigms for Materials and Science
- Biosynthesis of Emulsan Bioemulsifiers for Structure/Function and Biological Activation
- Functional Tissue Engineering
- Bioengineered Biomaterials
Selected Publications:
Mapping domain structures in silks from insects and spiders. E. Bini, D. Knight, D. L. Kaplan. J. Molecular Biology. 335:27-40 (2004).
Bone tissue engineering using human mesenchymal stem cells; effects of scaffold material and medium flow. Meinel, L., V. Karageorgiou, R. Fajardo, B. Snyder, V. Shinde-Patil, L. Zichner, D. L. Kaplan, R. Langer, G. Vunjak-Novakovic. Annals Biomed. Eng. 32:112-122 (2004).
Matrix-mediated retention of osteogenic differentiation potential by human adult bone marrow stromal cells during ex vivo expansion. J. R. Mauney, D. L. Kaplan, V. Volloch. Biomaterials 25:3233-3243 (2004).
Self-assembly and biocatlysis in the synthesis of nanostructured polymers and polymer-ceramic composites. C. Ford, V. John, G. McPherson, J. He, J. Akkara, D. L. Kaplan, A. Bose. In press (2004).
Osteogenic Differentiation of Human Bone Marrow Stromal Cells on Partially Demineralized Bone Scaffolds
In Vitro. Mauney JR, Blumberg J, Pirun M, Volloch V, Vunjak-Novakovic G, Kaplan DL
Tissue Engineering, 10:81-92 (2004).
Inflammatory responses to silk films in vitro and in vivo. L. Meinel, S. Hofmann, V. Karageorgiou, C. Kirker-Head, J. McCool, G. Gronwicz, L. Zichner, R. Langer, G. Vunjak-Novakovic. D. L. Kaplan.
Biomaterials. 26:147-155 (2004).
Nanoscale surface patterning of enzyme catalyzed polymeric conducting wires. P. Xu, D. L. Kaplan. Advanced Materials, 16:628-632(2004)
Porous 3D scaffolds from regenerated silk fibroin. R. Nazarov, H.-J. Jin, D. L. Kaplan.
Biomacromolecules. 5:718-726 (2004).
Biomaterials films of Bombyx mori silk with poly(ethylene oxide). H.-J. Jin, J. Park, U.-J. Kim, R. Valluzzi, P. Cebe, D. L. Kaplan.
Biomacromolecules. 5:711-717 (2004).
Structure and properties of silk hydrogels. U.-J. Kim, J. Park, C. Li, H.-J. Jin, R. Valluzzi, D. L. Kaplan.
Biomacromolecules. 5:786-792 (2004).
Microbial polysaccharides. T. Harrah, B. Panilaitis, D. L. Kaplan. In Microbial Polysaccharides, E. Rosenberg, Ed. (2004).
Mechanical stimulation promotes osteogenic differentiation of human bone marrow stromal cells on 3-D partially demineralized bone scaffolds in vitro. J. R. Mauney, S. Sjostorm, J. Blumberg, R. Horan, J. P. O’Leary, G. Vunjak-Novakovic, V. Volloch, D. L. Kaplan.
Calcified Tissue International, 74:458-468 (2004)
Bioreactors for orthopaedic tissue engineering. G. Vunjak-Novakovic, B. Obradovic, H. Madry, G. Altman, D. L. Kaplan. In Orthopaedic Tissue Engineering: Basic Science and Practice (Ed. A. I. Caplan and V. Goldberg), Marcel Dekker Inc., New York, pp. 123-147 (2004).
Impact of matrix trafficking by human fibroblasts. L. Abraham, J. Vorasi, D. L. Kaplan.
J. Biomedical Materials Research, 70A:39-48 (2004).
Tissue engineering of ligaments. G. Vunjak-Novakovic, G. Altman, R. Horan, D. L. Kaplan.
Annual Review of Biomedical Engineering. 6:14.1-14.26 (2004).
Tissue engineering of osteochondral plugs using human mesenchymal stem cells and silk scaffolds. Meinel, L., V. Karageorgiou, S. Hoffmann, R. Fajardo, B. Snyder, C. Li, L. Zichner, R. Langer, G. Vunjak-Novakovic, D. L. Kaplan.
Chemical Industry 58:68-69 (2004).
Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffolds. L. Meinel, S. Hoffmann, V. Karageorgiou, L. Zichner, R. Langer, G. Vunjak-Novakovic, D. L. Kaplan.
Biotechnology and Bioengineering. 88:379-391 (2004).
Human bone marrow stromal cell responses on electrospun silk fibroin mats. H. J. Jin, J. Chen, V. Kargeorgiou, G. H. Altman, D. L. Kaplan. Biomaterials 25:1039-1047 (2004).
Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffolds. L. Meinel, V. Kargeorgiou, S. Hofmann, R. Fajardo, B. Snyder, C. Li, L. Zichner, R. Langer, G. Vunjak-Novakovic, D. L. Kaplan.
J. Biomedical Materials Research, 71A:25-34 (2004).
Lessons from seashells: mineralized silica via protein templating, C. Wong Po Foo, J. Huang, D. L. Kaplan,
Trends in Biotechnology 22:577-585 (2004).
Vitamin C functionalized poly(methyl methacrylate) for free radical scavenging. A. Singh, D. L. Kaplan.
J. Macromolecular Science – Pure and Appl. Chemistry A41:1377-1386 (2004).
Horseradish peroxidase catalyzed polymerization of tyrosine derivatives for nanoscale surface patterning. P. Xu, D. L. Kaplan.
J. Macromolecular Science – Pure and Appl. Chemistry A41:1437-1446 (2004).
Emulsan quantitation by Nile Red quenching fluorescence assay, G. R. Castro, B. K. Larson, B. Panilaitis, D. L. Kaplan,
Applied Microbiology and Biotechnology, published online (2004).
Growth factor induced fibroblast differentiation from human bone marrow stromal cells in vitro. J. E. Moreau, J. Chen, D. Bramono, V. Volloch, H. Chernoff, G. Vunjak-Novakovic, J. Richmond, D. L. Kaplan, G. H. Altman.
J. Orthopaedic Research, 23: 164-174 (2005)
Mechanical properties of electrospun silk fibers. M. Wang, H.J. Jin, D. L. Kaplan, G. C. Rutledge.
Macromolecules, 37:6856-6864 (2004).
Matrix metalloproteinases and their clinical applications in orthopaedics. D. S. Bramono, D. L. Kaplan, J. C. Richmond, P. P. Weitzel, G. H. Altman. Clinical Orthopaedics. 428: 272-285 (2004).
Bone morphogenetic protein-2 decorated silk fibroin films induce osteogenic differentiation of human bone marrow stromal cells. V. Karageorgiou, L. Meinel, S. Hofmann, A. Malhotra, V. Volloch, D. L. Kaplan.
J. Biomedical Materials Research, 71A:528-537 (2004).
In vitro degradation of silk fibroin. R. L. Horan, K. Antle, A. L. Collette, Y. Wang, J. Huang, J. E. Moreau, V. Volloch, D. L. Kaplan, G. H. Altman.
Biomaterials 26:3385-3393 (2005).
In vitro and in vivo evaluation of differentially mineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering. J. Mauney, C. Jaquiery, V. Volloch, I. Martin, D. L. Kaplan.
Biomaterials, in press (2005).
Three dimensional aqueous-derived biomaterial scaffolds from silk fibroin. U.J. Kim, J. Park, H. J. Kim, M. Wada, D. L. Kaplan.
Biomaterials, in press (2005).
Tissue engineering of bone, S. Hofmann, D. L. Kaplan, G. Vunjak-Novakovic, L. Meinel. In Specialized cell cultures for tissue engineering, Eds. G. Vunjak-Novakovic, I. Freshney, J. Wiley, in press (2005).
Bioreactor cultivation of osteochondral tissues. Vunjak-Novakovic, G., L. Meinel, G. Altman, L. E. Freed, D. L. Kaplan. Orthodontics and Craniofacial Research, in press (2005).
Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cells. H. J. Kim, U-J. Kim , G. Vunjak-Novakovic, B-H. Min, D. L. Kaplan.
Biomaterials, in press (2005).
Emulsan quantitation by Nile Red quenching fluorescence assay, G. R. Castro, B. K. Larson, B. Panilaitis, D. L. Kaplan,
Applied Microbiology and Biotechnology, in press (2005).
Biopolymer scaffolds for tissue engineering. J. Velma, D. L. Kaplan. In Tissue Engineering, Advances in Biochemical Engineering/Biotechnology, Edited by K. Lee, D. L. Kaplan, Spring-Verlag, in press (2005).
The role of adult mesenchymal stem cells in bone tissue engineering applications: current status and future prospects. V. Karagerogiou, D. L. Kaplan.
Tissue Eng. in press (2005).
Courses Taught:
- ChBE 164 Biomaterials and Tissue Engineering
- ChBE 62/162 Molecular Biotechnology
- En 64 Introduction to Biomedical Engineering
- EN 3 Introduction to Biotechnology
- ChBE 163 Recombinant DNA Technologies
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